effects of l-carnitine on cardiac apoptosis in ischemic- reperfused isolated rat heart
نویسندگان
چکیده
carnitine is a vital biologic substance for transporting fatty acids into myocytes. it also facilitates fatty acids β-oxidation for energy production. in this study, effects of l-carnitine (l-car) on apoptosis in the ischemic isolated rat heart were investigated. male sprague-dawley rats were divided into four groups and anesthetized by sodium pentobarbital. the heart was removed and mounted on a langendorff apparatus then perfused by a modified krebs-henseleit (k/h) solution under a constant pressure at 37 °c. in the control group, the hearts were perfused only by normal k/h solution at stabilization, 30 min. regional ischemia and 120 min. reperfusion, while in each of the test groups, the hearts were perfused during ischemia-reperfusion with 0.5, 2.5 and 5 mm of l-car-enriched k/h solution, respectively. at the end of reperfusion, immunohistochemical detection of apoptotic cells was performed by using an in situ apoptosis detection kit. the number of tunel-positive cardiomyocytes was counted in five random high-power fields in each sample. in the control group, the number of apoptotic cells were 48±3 while addition of l-car (0.5, 2.5 and 5 mm) to the solution reduced the number of apoptotic cells to 6±1, 4±1 and 3±1, respectively (p
منابع مشابه
Effects of L-Carnitine on Cardiac Apoptosis in Ischemic- Reperfused Isolated Rat Heart
Carnitine is a vital biologic substance for transporting fatty acids into myocytes. It also facilitates fatty acids β-oxidation for energy production. In this study, effects of L-carnitine (L-Car) on apoptosis in the ischemic isolated rat heart were investigated. Male Sprague-Dawley rats were divided into four groups and anesthetized by sodium pentobarbital. The heart was removed and mount...
متن کاملEffects of Acetyl-L-Carnitine on Cardiac Arrhythmias and Infarct Size in Ischemic-Reperfused Isolated Rat Heart
Objective(s) This study aimed to examine whether acetyl-L-carnitine (ALC) was able to reduce cardiac arrhythmias and infarct size in the ischemic-reperfused isolated rat heart. Materials and Methods The isolated hearts were mounted on a Langendorff apparatus then perfused by a modified Krebs-Henseleit solution during 30 min regional ischemia and 120 min reperfusion (control) or by enriched K...
متن کاملeffects of acetyl-l-carnitine on cardiac arrhythmias and infarct size in ischemic-reperfused isolated rat heart
objective(s) this study aimed to examine whether acetyl-l-carnitine (alc) was able to reduce cardiac arrhythmias and infarct size in the ischemic-reperfused isolated rat heart. materials and methods the isolated hearts were mounted on a langendorff apparatus then perfused by a modified krebs-henseleit solution during 30 min regional ischemia and 120 min reperfusion (control) or by enriched kreb...
متن کاملEffects of Acetyl-L-Carnitine on Cardiac Arrhythmias and Infarct Size in Ischemic-Reperfused Isolated Rat Heart
Objective(s) This study aimed to examine whether acetyl-L-carnitine (ALC) was able to reduce cardiac arrhythmias and infarct size in the ischemic-reperfused isolated rat heart. Materials and Methods The isolated hearts were mounted on a Langendorff apparatus then perfused by a modified Krebs-Henseleit solution during 30 min regional ischemia and 120 min reperfusion (control) or by enriched Kreb...
متن کاملeffects of acute administration of acetyl-l-carnitine on hemodynamic function and infarct size in ischemic-reperfused isolated heart
متن کامل
Comparison between the effects of L-Carnitine and Acetyl-L-Carnitine on myocardial infarction size in ischemic heart
Introduction: In the present study, potential differences between the effects of L-carnitine (LC) and acetyl-Lcarnitine (ALC) on ischemia/reperfusion (I/R)-induced myocardial infarction size were investigated. Methods: Male Wistar rats were randomly divided into five groups and then anesthetized by sodium pentobarbital. Hearts of the animals were removed and quickly mounted on a Langendorff ...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of pharmaceutical sciencesجلد ۳، شماره ۱، صفحات ۱۹-۲۴
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023